YFMA0035
100μL
￥1100
WB， IHC， IF
Background：
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beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues. |
Product Information：
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Product name: Anti-beta Actin Mouse Monoclonal Antibody Isotype: IgG1 Form: Liquid Tested applications: WB, IHC-F, IHC-P, ICC/IF Dilution Ratio: WB: 1:5000-1:20000； IHC：1:50-1:200；IF: 1:50-1:200 Species reactivity: Human, Mouse, Rat, Monkey, Dog, Chicken, Hamster, Rabbit, Insect Host Species: Mouse Purity: Affinity purification |
Immunogen Information：
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Immunogen: Synthetic Peptide of β-actin Calculated molecular weight: 42kDa Observed molecular weight: 42kDa Swiss Prot: P60709 Gene ID (NCBI): 60 Alternative Names: ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta |
Storage：
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Services & Support：
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1、Please do not hesitate to contact us if you have any questions. 2、Please fill in the Technical Support Request Form and email the form to support@yfxbio.com within 3months of receipt of the goods, and we will response to your inquiry within 24 hours. 3、The after-sale service channel will be shut down after 3 months. 4、Producthasnot been fully validated for medical applications. For research use only. |
Application：
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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※ IF=10.717，Fang Chen, Qi Gao, Ai Wei et al. Histone deacetylase 3 aberration inhibits Klotho transcription and promotes renal fibrosis. Cell Death & Differentiation,28, pages1001–1012 (2021).
※ IF=4.441，Lanlan Gao, Qianqian Guo, Xiaoman Li et al. MiR-873/PD-L1 axis regulates the stemness of breast cancer cells. EBioMedicine. 2019 Mar:41:395-407.
※ IF=14.73，Jun Yin, Qun Wang, Shan Hou et al. Potent Protein Delivery into Mammalian Cells via a Supercharged Polypeptide. J. Am. Chem. Soc., 2018, 140 (49), pp 17234–17240.
※ IF=4.765，Lufeng Zheng, Zhiting Zhang, Shufang Zhang et al. RNA Binding Protein RNPC1 Inhibits Breast Cancer Cell Metastasis via Activating STARD13-Correlated ceRNA Network. Molecular Pharmaceutics. 2018 Jun 4;15(6):2123-2132.
※ IF=4.765，Xinwei Guo, Chenxi Xiang,Zhiting Zhang et al. Displacement of Bax by BMF Mediates STARD13 3′UTR-Induced Breast Cancer Cells Apoptosis in an miRNA-Depedent Manner. Mol Pharm. 2018 Jan 2;15(1):63-71.
※ IF=3.414，Wei Wang, Hao Wu, Hongli Yu et al. Typhonium giganteum Lectin Exerts A Pro-Inflammatory Effect on RAW 264.7 via ROS and The NF-kB Signaling Pathway. Toxins (Basel). 2017 Sep 7;9(9):275.
※ IF=4.938，Jun Yang, Shasha Yang, Fangfang Bi et al. TIMAP repression by TGF βand HDAC3-associated Smad signaling regulates macrophage M2 phenotypic phagocytosis. J Mol Med (Berl). 2017 Mar;95(3):273-285.
