2× SYBR Green Fast qPCR Master Mix

产品简介

本产品含有优化浓度的热启动Taq DNA Polymerase、SYBR Green I、dNTPs、Mg2+、反应缓冲液和稳定剂等成分。

试剂中的热启动Taq DNA Polymerase高温加热前，抗Taq单克隆抗体与Taq结合，抑制Taq的聚合酶活性，从而抑制在低温条件下出现的由引物和模板 DNA 非特异性杂交或引物二聚体引起的非特异性扩增。抗体在PCR反应的预变性步骤中已完全失活，不会阻碍之后Taq Polymerase反应，大大提高了 PCR 反应的灵敏度及特异性。

试剂中SYBR Green I优化了浓度，渗入DNA双链后，荧光信号增强，而没有渗入DNA双链的SYBR Green I荧光信号不变，从而保证荧光信号的增加与PCR产物的增加同步，荧光可以在退火或延伸阶段测定。

产品特点

本产品为2×预混实时荧光定量快速PCR反应体系，使用时只需加入模板、引物和水，使其工作浓度为1×，即可进行反应。具有快速简便、灵敏度高、特异性强、稳定性好等优点，可最大限度地减少人为误差、节约PCR实验操作时间、降低污染几率。

注意事项

1. 使用前请上下颠倒轻轻混匀，尽量避免起泡，并经过短暂离心后使用。

2. 保存或者使用时应避免强光照射。

3. 避免反复冻融本产品。

应用文献

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